Reporter Plasmids
In Stock Reporter Plasmids
Aldevron, your trusted partner since 1998, has several reporter plasmids available.
pALD-CV77-Luciferase Reporter
Consistent with the pALD-CV77 cloning backbone, Aldevron offers a pre-made reporter construct, pALD-CV77-Luciferase, which has several advantages:
- Commercially available sequence for research through clinical use
- Kanamycin resistance
- Consistent, reliable supply
- Immediately available at research grade
- Available in 100ug free sample vials, 5mg vials and 10mg vials, research grade only
gWiz Reporter Plasmids
The gWiz plasmids are designed for a wide range of mammalian cells and tissues. They contain a modified promoter, followed by intron A from the CMV immediate early gene, and an artificial transcription terminator. These ready-to-use plasmids can be applied directly to most in vitro and in vivo applications.
Each gWiz plasmid is ready to ship stocked at 5mg/mL in water enabling you to easily adjust to the buffer of your choice. Common features of the gWiz plasmids include:
- In vitro or in vivo gene expression
- CMV IE promoter
- Intron A
- Artificial transcription terminator
- Kanamycin resistance
- Available in 5mg and 10mg vials, research grade only
Quality & Ordering Information
pALD-CV77 Luciferase and gWiz Quality Control Specifications
| pALD-CV77-Luciferase | gWiz Reporter Plasmids | |
| Appearance | Clear and Colorless | Clear and colorless |
| Buffer | TE | Water |
| Bioburden | No growth in 48 hours at 37 +/- 2 C | N/A |
| Concentration | 1.0mg/mL +/- 10% | 5.0 mg/mL +/- 10% |
| DNA Homogeneity | ≥80% Supercoiled | Predominantly supercoiled |
| Endotoxin | ≤100 EU/mg | ≤ 100 EU/mg |
| Identity | Confirmed Against DNA Ladder | Confirmed Against DNA Ladder |
| Plasmid Identity | Identical to Reference Sequence | N/A |
| ABS 260/280 Ratio Purity | 1.80 – 2.00 | 1.80 - 2.00 |
| Residual Host Genomic DNA via qPCR | ≤5% | ≤ 5% |
| Residual Host Protein via BCA | ≤2% | Not visible at 200 ng load |
| Residual Host RNA via SYBR Gold | ≤5% by Semi-Quantitative Densitometry | N/A |
| Restriction Digest | Matches Expected Restriction Pattern | N/A |
| Mycoplasma Contamination | Negative for the Presence of Mycoplasma | N/A |
| Residual Host RNA | N/A | Not visible at 200 ng load |
<table class="table_order"><thead><tr><th>Description</th><th>Cat #</th><th>Price</th><th>Order</th></tr></thead><tbody><tr><td><p><strong class="item_title">pALD-CV77-Luciferase 10 mg</strong></p></td><td>5078-10</td><td><div class="pricetable mmg-collapsible"><strong class="module-title">pALD-CV77-Luciferase 10 mg Pricing</strong><div id="pricetable_112851" class="content" style="display:none"> <table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody><tr><td>1</td><td>$1080.00</td></tr><tr><td>2</td><td>$935.00</td></tr><tr><td>3</td><td>$860.00</td></tr><tr><td>4</td><td>$845.00</td></tr><tr><td>5+</td><td>$804.00</td></tr></tbody></table></div></div></td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue= https://main--aldevron--hlxsites.hlx.page/catalog-products/pald-cv77-luciferase" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" style="width:75px" name="buyid"><option value="112851">10 mg</option></select><br><button class="addtocart" type="submit">Add to Cart</button></form></td></tr><tr><td><p><strong class="item_title">pALD-CV77-Luciferase 5 mg</strong></p></td><td>5078-5</td><td><div class="pricetable mmg-collapsible"><strong class="module-title">pALD-CV77-Luciferase 5 mg Pricing</strong><div id="pricetable_112850" class="content" style="display:none"> <table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody><tr><td>1</td><td>$590.00</td></tr><tr><td>2</td><td>$540.00</td></tr><tr><td>3</td><td>$500.00</td></tr><tr><td>4</td><td>$467.50</td></tr><tr><td>5+</td><td>$446.00</td></tr></tbody></table></div></div></td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue= https://main--aldevron--hlxsites.hlx.page/catalog-products/reporters/traditional-pdna-reporters" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" style="width:75px" name="buyid"><option value="112850">5 mg</option></select><br><button class="addtocart" type="submit">Add to Cart</button></form></td></tr><tr><td class="labelcell"><strong class="item_title">gWiz-GFP</strong><p><a href="https://606687.secure.netsuite.com/core/media/media.nl?id=1594&c=606687&h=4f1ad11f91d7b22e1c9a" target="_blank" class="button zoom-map cboxElement" rel="noopener" onclick='_gaq.push(["_trackEvent","Button","Map","5006/5007 gWiz-GFP"])'>MAP</a></p><div class="pricetable mmg-collapsible"><strong class="module-title">QUANTITY PRICING</strong><div id="pricetable_56373" class="content" style="display:none">Cat# 5007 Only<table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody><tr><td>1</td><td>$1080.00</td></tr><tr><td>2</td><td>$935.00</td></tr><tr><td>3</td><td>$860.00</td></tr><tr><td>4</td><td>$845.00</td></tr><tr><td>5+</td><td>$804.00</td></tr></tbody></table></div></div><table class="table_orderNested"><tbody><tr><td><span class="info_label">Transgene</span></td><td>Green Fluorescent Protein</td></tr><tr><td><span class="info_label">Plasmid Size</span></td><td>5757 bp</td></tr><tr><td><span class="info_label">Notes</span></td><td>GFP expression in mammalian cells and tissues</td></tr></tbody></table></td><td>5006<br>5007</td><td><span class="price" id="price_56482">$590.00</span>- 5 mg<br><span class="price" id="price_56373">$1,080.00</span>- 10 mg</td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue= https://main--aldevron--hlxsites.hlx.page/catalog-products/dnas/gwiz" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" name="buyid"><option value="56482">5 mg</option><option value="56373">10 mg</option></select><br><button type="submit" class="addtocart" onclick='trackCart("5006/5007 gWiz-GFP",this.parentNode.elements.buyid,this.parentNode.elements.qty.value)'>Add to Cart</button></form></td></tr><tr><td class="labelcell"><strong class="item_title">gWiz-Luc</strong><p><a href="https://606687.secure.netsuite.com/core/media/media.nl?id=706&c=606687&h=38863950c295c63ad752" target="_blank" class="button zoom-map cboxElement" rel="noopener" onclick='_gaq.push(["_trackEvent","Button","Map","5000/5001 gWiz-Luc"])'>MAP</a></p><div class="pricetable mmg-collapsible"><strong class="module-title">QUANTITY PRICING</strong><div id="pricetable_56370" class="content" style="display:none">Cat# 5001 Only<table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody><tr><td>1</td><td>$1080.00</td></tr><tr><td>2</td><td>$935.00</td></tr><tr><td>3</td><td>$860.00</td></tr><tr><td>4</td><td>$845.00</td></tr><tr><td>5+</td><td>$804.00</td></tr></tbody></table></div></div><table class="table_orderNested"><tbody><tr><td><span class="info_label">Transgene</span></td><td>Luciferase</td></tr><tr><td><span class="info_label">Plasmid Size</span></td><td>6732 bp</td></tr><tr><td><span class="info_label">Notes</span></td><td>Luc expression in mammalian cells and tissue</td></tr></tbody></table></td><td>5000<br>5001</td><td><span class="price" id="price_56479">$590.00</span>- 5 mg<br><span class="price" id="price_56370">$1,080.00</span>- 10 mg</td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue= https://main--aldevron--hlxsites.hlx.page/catalog-products/dnas/gwiz" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" name="buyid"><option value="56479">5 mg</option><option value="56370">10 mg</option></select><br><button type="submit" class="addtocart" onclick='trackCart("5000/5001 gWiz-Luc",this.parentNode.elements.buyid,this.parentNode.elements.qty.value)'>Add to Cart</button></form></td></tr><tr><td class="labelcell"><strong class="item_title">gWiz-blank</strong><p><a href="https://606687.secure.netsuite.com/core/media/media.nl?id=1592&c=606687&h=9c668159252a11acd146" target="_blank" class="button zoom-map cboxElement" rel="noopener" onclick='_gaq.push(["_trackEvent","Button","Map","5008/5009 gWiz-blank"])'>MAP</a></p><div class="pricetable mmg-collapsible"><strong class="module-title">QUANTITY PRICING</strong><div id="pricetable_56374" class="content" style="display:none">Cat# 5009 Only<table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody><tr><td>1</td><td>$1080.00</td></tr><tr><td>2</td><td>$935.00</td></tr><tr><td>3</td><td>$860.00</td></tr><tr><td>4</td><td>$845.00</td></tr><tr><td>5+</td><td>$804.00</td></tr></tbody></table></div></div><table class="table_orderNested"><tbody><tr><td><span class="info_label">Transgene</span></td><td>none</td></tr><tr><td><span class="info_label">Plasmid Size</span></td><td>5100 bp</td></tr><tr><td><span class="info_label">Notes</span></td><td>Multiple cloning site for inserting gene of interest; Expression in mammalian cells and tissue</td></tr></tbody></table></td><td>5008<br>5009</td><td><span class="price" id="price_56483">$590.00</span>- 5 mg<br><span class="price" id="price_56374">$1,080.00</span>- 10 mg</td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue= https://main--aldevron--hlxsites.hlx.page/catalog-products/reporters/traditional-pdna-reporters" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" name="buyid"><option value="56483">5 mg</option><option value="56374">10 mg</option></select><br><button type="submit" class="addtocart" onclick='trackCart("5008/5009 gWiz-blank",this.parentNode.elements.buyid,this.parentNode.elements.qty.value)'>Add to Cart</button></form></td></tr></tbody></table>