Nanoplasmid™ Reporter Constructs
Discover the power of small with Nanoplasmid Reporter Constructs
Nanoplasmid is a next-generation plasmid vector designed for cell and gene therapy applications. Benefits include improved transfection efficiencies, reduced post-transfection toxicity, superior transgene expression, and reduced silencing through its small backbone design (<500bp).
You can experience the power of Nanoplasmid by requesting a free reporter construct below.
Experiments comparing nanoplasmids to traditional plasmids conducted with tissue cells in vitro and cardiomyocyte heart tissues in vivo have shown a significant increase in gene expression while using lower voltages during electroporation.
Figure 1. Boye, C., Arpag, S., Francis, M., DeClemente, S., West, A., Heller, R., & Bulysheva, A. (2022). Reduction of plasmid vector backbone length enhances reporter gene expression. Bioelectrochemistry, 144, 1-8Δ
Do you have questions about Nanoplasmids? Read our frequently asked questions.
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<th><strong>Constructs*</strong> </th>
<th> <strong> Description </strong> </th>
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<td>pALD-Nanoplasmid-CMV-eGFP</td>
<td>Nanoplasmid construct using the cytomegalovirus (CMV) promotor and the enhanced green fluorescent protein reporter.</td>
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<td>pALD-Nanoplasmid-CMV-Luc</td>
<td>Nanoplasmid construct using the cytomegalovirus (CMV) promotor and a luciferase (Luc) reporter.</td>
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<td>pALD-Nanoplasmid-CAG-eGFP</td>
<td>Nanoplasmid constuct using the hybrid cytomegalovirus (CMV) enhancer fused to the chicken beta-actin promotor with a enhanced green fluorescent protein reporter.</td>
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<td>pALD-Nanoplasmid-CAG-Luc</td>
<td>Nanoplasmid construct using the hybrid cytomegalovirus (CMV) enhancer fused to the chicken beta-actin promotor with a luciferase (Luc) reporter.</td>
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<td>pALD-Nanoplasmid-EF1a(intron)-eGFP</td>
<td>Nanoplasmid construct using Elongation factor 1 (EF1) with an intronic sequence with an enhanced green fluorescent protein (EGFP) reporter.</td>
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<td>pALD-Nanoplasmid-EF1a(intron)-Luc</td>
<td>Nanoplasmid construct using Elongation factor 1 (EF1) with an intronic sequence with a luciferase reporter.</td>
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<td>pALD-Nanoplasmid-EF1S-eGFP</td>
<td>Nanoplasmid construct using Elongation factor 1 (EF1) short sequence with the enhanced green fluorescent protein (EGFP) reporter.</td>
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<td>pALD-Nanoplasmid-EF1S-Luc</td>
<td>Nanoplasmid construct using Elongation factor 1 (EF1) short sequence with a luciferase reporter.</td>
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</table>
*100 µgs per vial (concentration is 2mg/mL) in endotoxin-free water.
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ΔReprinted from Bioelectrochemistry, Vol 144, Boye C, Arpag S, Francis M, DeClemente S, West A, Heller R, Bulysheva A., Reduction of plasmid vector backbone length enhances reporter gene expression, Pages No.107981, Copyright (2022), with permission from Elsevier.